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Basic Info.
Model NO.
FZ008BF2
Sensitivity
Minimum:100 Cfu/Test
Feature
Easy to Operate
Customized Support
OEM
Transport Package
Carton
Specification
48tests/box
Trademark
BHK
Origin
Guangdong, China
Product Description
Introduction
This kit is only suitable for the qualitative detection of Vibrio parahaemolyticus in food. Other tests shall be carried out in accordance with the national standard "GB4789.7-2013 National Food Safety Standard Food Microbiology Test Vibrio parahaemolyticus
Test".
Test".
Principle
Based on Real Time PCR technology, primers, fluorescent probes and other reagents required for the specific gene of Vibrio parahaemolyticus are used, and the sample to be tested is added to carry out the amplification reaction. During the amplification process, the fluorescent probe binds to the target gene fragment, which can be decomposed by Taq enzyme and produce a fluorescent signal. At this time, the fluorescent quantitative PCR instrument can identify the fluorescent signal, and draw the corresponding real-time amplification curve according to its strength change, and then determine whether Vibrio parahaemolyticus is detected.
How to use?
1. Sample pretreatment 1) Preparation of sample enrichment solution template DNA: a) Take 25 g (mL) of the sample to be tested, cut it into pieces, place it in 225 mL of 3% sodium chloride alkaline peptone water, and pre-enrich it at 36 ±1 for 8~18 h; b) Take 1 mL of the above enrichment solution into a 1.5 mL sterile centrifuge tube, centrifuge it at 6000 r/min for 5 min, and completely remove the supernatant; c) Add 30 μL of lysis buffer to fully suspend the sediment at the bottom of the tube, tap the tube wall to eliminate bubbles, and heat at 99 for 10 min; d) Centrifuge at 12000 r/min for 15 min. The supernatant is the sample DNA to be tested. Let it stand on ice. If it is not used for a long time, it should be centrifuged again. 2) Preparation of template DNA of suspicious colonies: Pick suspicious colonies, fully suspend them in a sterile centrifuge tube pre-added with 30 μL of lysis buffer, and then follow the above steps c) and d). 2. Sample addition and reaction 1) Take n PCR reaction tubes as required (n=1 tube of negative control + number of samples to be tested + 1 tube of positive control), take out the premix from the kit, fully melt, vortex and briefly centrifuge, add 20 μL of premix to each tube and set aside. 2) Add 5 μL of negative control, sample DNA to be tested, and positive control to the above n reaction tubes, respectively, with a total reaction volume of 25 μL. Cover the tube cap tightly, centrifuge briefly, and immediately perform PCR amplification reaction. 3) The PCR reaction system is 25 μL, take the FAM detection channel, and collect the fluorescence signal at 60°C in reaction stage 2. The specific procedure is as follows:
1. Sample pretreatment 1) Preparation of sample enrichment solution template DNA: a) Take 25 g (mL) of the sample to be tested, cut it into pieces, place it in 225 mL of 3% sodium chloride alkaline peptone water, and pre-enrich it at 36 ±1 for 8~18 h; b) Take 1 mL of the above enrichment solution into a 1.5 mL sterile centrifuge tube, centrifuge it at 6000 r/min for 5 min, and completely remove the supernatant; c) Add 30 μL of lysis buffer to fully suspend the sediment at the bottom of the tube, tap the tube wall to eliminate bubbles, and heat at 99 for 10 min; d) Centrifuge at 12000 r/min for 15 min. The supernatant is the sample DNA to be tested. Let it stand on ice. If it is not used for a long time, it should be centrifuged again. 2) Preparation of template DNA of suspicious colonies: Pick suspicious colonies, fully suspend them in a sterile centrifuge tube pre-added with 30 μL of lysis buffer, and then follow the above steps c) and d). 2. Sample addition and reaction 1) Take n PCR reaction tubes as required (n=1 tube of negative control + number of samples to be tested + 1 tube of positive control), take out the premix from the kit, fully melt, vortex and briefly centrifuge, add 20 μL of premix to each tube and set aside. 2) Add 5 μL of negative control, sample DNA to be tested, and positive control to the above n reaction tubes, respectively, with a total reaction volume of 25 μL. Cover the tube cap tightly, centrifuge briefly, and immediately perform PCR amplification reaction. 3) The PCR reaction system is 25 μL, take the FAM detection channel, and collect the fluorescence signal at 60°C in reaction stage 2. The specific procedure is as follows:
| Reaction stage | Temperature | Time | Signal collection | Number of cycles |
| 1 | 95°C | 30 sec | / | 1 |
| 2 | 95°C | 5 sec | / | } 40 |
| | 60°C | 30 sec | | |
Note: Collect fluorescence signals at 60 in reaction stage 2. For multi-channel fluorescence PCR instrument, select fluorescein "FAM" as the signal collection channel, select "None" for quenching group, and select "None" for dye correction. 3. Results: In general, the test results can be directly read through the baseline and threshold automatically set by the software. If adjustment is required, it can be adjusted according to the instrument's own conditions (such as noise, etc.) and the different selected fluorescence channels. 1) Quality control: The negative control does not show an obvious S-type amplification curve or the Ct value is greater than 35, and the positive control shows an S-type amplification curve and its Ct value is less than 30. If the positive and negative controls do not meet the above conditions at the same time, the test results are invalid and should be retested or contact product technical support. 2) Result interpretation: (interpretation based on the Ct value obtained by the reaction, see the table below for details:)
| Ct value | | Result interpretation | | | |||
| ≤35 | | Vibrio parahaemolyticus is positive | | | |||
| 35~37 | | It is recommended to retest. If the result Ct ≥ 37, then Vibrio parahaemolyticus is negative, otherwise it is positive for Vibrio parahaemolyticus. | | | |||
| ≥37 | | Vibrio parahaemolyticus is negative. | | | |||
Our service
1.Offering Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
To better ensure the safety of your goods, professional, environmentally friendly, convenient and efficient packaging services will be provided.
Guangdong HuanKai Microbial Sci. & Tech. Co.,Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and other industry.
1. Who are we?
HuanKai are based in Guangdong, China, start from 1993,there are total about 301-500 people in HKM. HuanKai has successfully established four major product lines: microbiological testing reagents and consumables, digital laboratory instruments, rapid water testing products for physical and chemical analysis, and eco-friendly cleaning and disinfection solutions. These lines boast over 2,000 different products, each protected by independent intellectual property rights.
2. How can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;
3.What can you buy from us?
Microbial Detection Product,Peptone&Tryptone,Deydrated Culture Media,Granular Media,Microbial Air Sampler and so on.
4. Why should you buy from us not from other suppliers?
1.With strong R&D capacity and good scientific research conditions,over 30 years of experience 2.One of the largest physicochemical reagent and culture media manufactuer in China follow international standard 3.Stable high quality main raw material from Europe & strictly quality control
5. What services can we provide?
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Cash,Escrow;
Language Spoken:English,Chinese