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Basic Info.
Model NO.
P122H
Specifications
32 Tests/Kit
Validity
12 months
Transport Package
Paper Carton with Icy Bag
Specification
44*44*45 cm
Trademark
Tianlong
Origin
Suzhou, China
HS Code
3822009000
Production Capacity
5000000 Test/Year
Product Description
Product Name:
Influenza A/B Virus RNA Detection Kit (Fluorescence PCR Method)
Spec:
32T/kit
Intended Use:
This kit is used for qualitative detection of influenza A virus RNA and Influenza B virus RNA in human oropharyngeal swab sample.
Influenza viruses include type A, B and C, with Type A being the most likely to cause epidemics, followed by Type B, and type C rarely causing epidemics. Influenza A virus can be divided into 16 H subtypes (H1~H16) and 9 N subtypes (N1~N9) according to the virus particle envelope hemagglutinin (HA) and antigenicity of neuraminidase (NA) proteins. So far, it has been reported H1, H2, H3, H5, H7 and H9 subtypes infection in human body. Influenza B virus is not classified, but there are mainly two lineages -Yamagata (Y) and Victoria (V). After influenza virus infection, the human body will experience headache, fever, body ache, nasal congestion, sore throat and other symptoms.
This kit is suitable for patients with influenza virus characteristics and those who have been in close contact with influenza patients. When the kit is used, operators must have been professionally trained in nucleic acid amplification or molecular biological method detection, have relevant experimental operation qualification. The laboratory should have reasonable biosafety preparedness facilities and protection procedures.
Principle of Test:
The highly conserved sequences in MP gene of influenza A virus and NP gene of influenza B virus were selected as target regions, and specific primers and TaqMan fluorescent probes were designed. The probes can specifically bind to a nucleic acid template in the middle of the primer amplification region. In the process of PCR extension reaction, the exonuclease activity of Taq enzyme will cut off the 5'end fluorescent group from the probe, freeing it in the reaction system, thus breaking away from the shielding of the 3'end fluorescence quenching group, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of influenza A/B virus nucleic acid in closed reaction system.
The internal standard of the kit was a pair of housekeeper gene specific primers combined with specific probes. The probe can specifically bind to a nucleic acid template in the middle of the primer amplification region, and the internal standard adopts the Texas_Red channel, so as to realize the monitoring of the detection process in the closed reaction system, and effectively monitor the occurrence of false negative.
Storage Condition & Validity:
All reagents could be stored at -20ºC±5ºC for 12 months.
The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transit time should not exceed 7 days.
The unused reagents can be sequentially cryopreservation without affecting their stability. The regents could be preserved for 30 days at -20ºC±5ºC after repeated thawing and freezing three times. The opened reagents should be placed at room temperature under the condition of no more than 8 hours. The products should be transport by ice box or refrigerated truck. Simulated transport tests indicate that the stability and validity could not be influenced by transport, but the transportation time should not exceed 7 days.
Applicable Instrument:
ABI 7500 Real-Time PCR System , Tianlong Gentier96E and Gentier96R PCR analysis system
Sample Requirements:
1. Sample: human oropharyngeal swab sample
2. Sampling apparatus disposable oropharyngeal swab approved by the State shall be used.
3. Preservation solution: sterile saline is recommended.
4. Collection: for specific sampling method, please refer to the "Microbial Specimen Collection Manual".
5. Storage: Samples can be stored at 2~8ºC for no more than 24 hours; under -20ºC for no more than 3 months; under -70ºC for a long time, but repeated freeze-thaw should be avoided. The sample extracts can be stored at 2~8ºC for no more than 12 hours; under -20ºC for no more than 3 months; under -70ºC for at least 7 months, the longer storage time needs to be verified, but repeated freeze-thaw should be avoided.
6. Transportation: Refrigerated and sealed type transportation with foam box.
| Product Name | Influenza A/B Virus RNA Detection Kit (Fluorescence PCR Method) |
| Cat.No | P122H |
| Specification | 32T/Kit |
| Specimen | Oropharyngeal swabs, nasopharyngeal swab, sputum and alveolar lavage fluid |
| Sensitivity | 500 copies/mL |
| Precision | ≤5.0% |
| Storage & Validity | -25ºC~-15ºC for 12 months |
| Applicable Equipment | Instruments with FAM, VIC (HEX), Cy5 channels such as Applied Biosystems™ 7500 Real-Time PCR Systems and Tianlong Gentier Real-time PCR Systems |
| Certificate | CE marked |
