• Canine Cryptosporidium Antigen Rapid Test 
• INTENDED USE
• Cryptosporidium is a common intestinal parasite that affects not only dogs, cats, cattle, but also human beings. It can cause symptoms like diarrhea, vomiting, gas, and abdominal discomfort in animals. The Canine Cryptosporidium Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Cryptosporidium antigen (Crypto Ag) in dog's feces or vomit specimen.
  Assay Time:  5-10 minutes
• 
• PRINCIPLE
• The Canine Cryptosporidium Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is Cryptosporidium antigen in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Cryptosporidium antigen in the specimen.
• 
• REAGENTS AND MATERIALS
• Test devices, with disposable droppers
• Assay buffer 
• Cotton swabs
• Products Manual
• STORAGE AND STABILITY
• The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (24 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
• TEST PROCEDURE
• Collect dog's fresh feces or vomit with the cotton swab from dog's anus or from the ground. 
• Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
• Take out the test device from the foil pouch and place it horizontally.
• Suck the treated sample extraction from the assay buffer
• tube and place 4 drops into the sample hole "S" of the test device.
• Note: If the liquid will not flow through the surface of the test strip in 30 seconds, please add another drop of the treated sample extraction.
• Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

 

• INTERPRETATION OF RESULTS
• Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
• Negative (-): Only clear C line appear. No T line.
• Invalid: No colored line appears in C zone. No matter if T line appears.
• PRECAUTIONS
• All reagents must be at room temperature before running the assay.
• Do not remove test cassette from its pouch until immediately before use.
• Do not use the test beyond its expiration date.
• The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
• All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
• LIMITATION
• The Canine Cryptosporidium Antigen Rapid Test is for in vitro veterinary diagnosis use only. All result should be considered with other clinical information available with veterinarian. It is suggested to apply a further confirmative method such as PCR when positive result was observed.

Hangzhou Immuno Biotech Co., Ltd is the original organization in IMMNUO Group, which was established in 2014. The team of Hangzhou Immuno Biotech has developed a series of proteins and rapid test kits for the in vitro diagnostic industry in early stage. Gradually, IMMUNO was well known as a good R&D partner and good supplier of veterinary rapid test products. With great patience and continuous investment in design and development of IVD relative reagents and test kits, we got several encouraging achievements in the past year, especially in veterinary diagnostic field.

IMMUNO has participated in the fighting with the epidemic and made a contribution. With a deep reflection, IMMUNO has realized the possibility of potential pandemic of zoonosis in future between human beings and animals. Diseases between human and animal are important projects what we are familiar with and then we decided to persue the "One Health" philosophy from then on.

To achieve that goal, IMMUNO has decided to focus on the development of rapid testing tools for zoonosis diseases and formed a joint action within our group. Now, we have a scientific team to analyse and develop recombinant proteins and antibodies, a technical team to apply the proteins and antibodies to immunoassay format, and also a joint team to study the diseases themselves and their possible application scenarios.

Hangzhou Immuno Biotech Co., Ltd. will focus on the human medical diagnostic field and mainly cover the following directions: rapid tests for vector-borne diseases(VBDs), rapid tests for sexually transmitted diseases (STDs), rapid tests for respiratory system diseases and rapid tests for digestive system diseases. Besides, with strong R&D capability, we would pay more attention to the diagnosis of neglected tropical diseases (NTDs).

IMMUNO will continuously contribute to the development of diagnostic tools for the whole human society and nature world.